首页期刊介绍编委会投稿须知电子期刊广告合作联系我们下载专区留言板

?《中国期刊网》《中国学术期刊(光盘版)全文收录》《中国学术期刊综合评价数据库》来源期刊 “万方数据-数字化期刊群”全文上网《中国核心期刊(遴选数据库)收录》《中文科技期刊数据库》来源期刊《龙源期刊网》合作期刊??? 友情提醒:我刊未委托任何单位、网站或个人代理征稿,以免上当受骗。

位置:首页 >> 电子期刊 >> 正文
儿童肺炎支原体肺炎外周血miRNA-492的表达
作者:孙旦1?李玉琴2?丁莹2?雷小丽2?骆亚丽2?周卫芳2▲?
单位:1.江苏省太仓市沙溪人民医院儿科?太仓215400?2.苏州大学附属儿童医院儿科?苏州市?215123)?
关键词:肺炎支原体肺炎?miRNA-492?TNF-α?IL-6?HMGB1?
分类号:R725.6
出版年,卷(期):页码:2019,47(6):427-431
摘要:

目的 探讨miRNA-492在儿童支原体肺炎中的作用,进一步了解支原体感染的免疫致病机制。方法 选取2014年3月至2015年6月期间住院治疗,确诊为肺炎支原体肺炎患儿23例作为实验组(MPP组),同时期健康儿童20例作为对照组(NC组),采用ELISA法检测TNF-α、IL-6,RT-qPCR法检测HMGB1及miRNA-492的表达量,分析两组实验室检查结果、免疫球蛋白水平及淋巴细胞亚群分布,探讨miRNA-492与TNF-α、IL-6、HMGB1及其他实验室指标的相关性。结果 肺炎支原体肺炎组患儿外周血miRNA-492、TNF-α、IL-6、HMGB1的表达水平均高于健康对照组(P<0.05)。MPP组miRNA-492与Neut、Lymph、CRP、AST、LDH、HBDH、PA、IgA、IgG、CD3+CD4+百分比、NK 细胞百分比、CD19+CD23+百分比、TNF-α、IL-6均无明显相关性(P>0.05),与HMGB1呈负相关(P<0.01)。结论 miRNA-492、TNF-α、IL-6及HMGB1在肺炎支原体肺炎感染和发病中起一定作用,作用机制可能不同。
?

Objective To investigate the role of miRNA-492 in children with mycoplasma pneumoniae pneumonia and to further understand the immune pathogenic mechanism of mycoplasma infection.Methods 23 children diagnosed with mycoplasma pneumonia during hospitalization from March 2014 to June 2015 were selected as experimental group(MPP group),and 20 healthy children in the same period were selected as control group(NC group).And then,TNF-α and IL-6 were detected by ELISA,and the expression of HMGB1 and miRNA-492 by RT-qPCR.The results of laboratory examination,immunoglobulin levels and the distribution lymphocyte of subsets in the two groups were analyzed.In addition,correlation between miRNA-492 and TNF-α,IL-6,HMGB1 and other laboratory indexes was explored.Results The expressions of microRNA492,TNF-α,IL-6 and HMGB1 in peripheral blood of children with mycoplasma pneumoniae pneumonia were higher than those of healthy control group(P<0.05).In the MPP group,miRNA-492 was not obviously correlated with TNF-α,IL-6,Neut,Lymph,CRP,AST,LDH,HBDH,PA,IgA,IgG and the percentages of CD3+CD4+,NK cell and CD19+CD23+(P>0.05),but it was negatively correlated with HMGB1(P<0.01).Conclusion MiRNA-492,TNF-α,IL-6 and HMGB1 play certain roles in the immune pathogenesis of mycoplasma pneumoniae pneumonia,but their mechanisms of action may be different.

基金项目:
苏州市科技计划项目(NO.SS 201535)
作者简介:
参考文献:
服务与反馈:
文章下载】【加入收藏

?右江民族医学院附属医院《右江医学》编辑部版权所有 CopyRight:www.yjyx.org All Rights Reserved.
本网站所刊登的各种新闻﹑信息、材料和各种稿件,版权均归本编辑部所有,未经协议授权,禁止转载、禁止下载使用。
本网站由右江民族医学院附属医院《右江医学》编辑部及博科思网络技术(北京)有限公司共同维护
编辑部电话/传真:0776-2825575;网站技术支持电话:010-68713668-9088
地址:广西百色市中山二路18号,邮编:533000
备案号:桂ICP备06012708号-3 ???? 公安局备案号:45100202000267号